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Contents 1 Parts 1.1 BBa_K363001 1.2 BBa_K363006 1.2.1 Usage and Biology 1.3 BBa_K363002 1.4 BBa_K363003 2 =

Parts

BBa_K363001

The consensus calcium dependent response element binding site for the Crz1 activator.

This part is a binding site for the Crz1 transcriptional activator. When placed upstream of a eukaryotic promoter sequence, this sequence results in the calcium dependant transcription of downstream coding sequences given that Crz1 and the upstream signal transducing phosphotase calcineurin are constitutively expressed. The signal cascade occurs when calcineurin binds to cytosolic calcium, activating its phosphotase activity. The active calcineurin dephosphorylates cytoplasmic Crz1, resulting in translocation of Crz1 to the nucleus where it binds to this part and promotes the assembly of general transcription factors at the promoter sequence.

BBa_K363006

A characterized calcium dependent response element binding site for the Crz1 activator

This part is a binding site for the Crz1 transcriptional activator. When placed upstream (~700 bp in the yeast gene FKS2/GSC2) of a eukaryotic promoter sequence, this sequence results in the calcium dependant transcription of downstream coding sequences given that Crz1 and the upstream signal transducing phosphotase calcineurin are constitutively expressed. The signal cascade occurs when calcineurin binds to cytosolic calcium, activating its phosphotase activity. The active calcineurin dephosphorylates cytoplasmic Crz1, resulting in translocation of Crz1 to the nucleus where it binds to this part and promotes the assembly of general transcription factors at the promoter sequence.

Usage and Biology

This part was characterized as part of a larger sequence (see design notes), as it occurs naturally as an activator of the yeast gene FKS2, which is codes for an enzyme involved in spore wall synthesis. We found that applying a voltage of 8V for 40-80 seconds provided maximal induction using our apparatus. This apparatus was, roughly speaking, a cylindrical coaxial electrode that was about .75cm across. We used SC media. For full details, see the 2010 Johns Hopkins Team wiki.

BBa_K363002

A calcium dependent response element binding site for the Crz1 activator

This part is a binding site for the Crz1 transcriptional activator. When placed upstream of a eukaryotic promoter sequence, this sequence results in the calcium dependant transcription of downstream coding sequences given that Crz1 and the upstream signal transducing phosphotase calcineurin are constitutively expressed. The signal cascade occurs when calcineurin binds to cytosolic calcium, activating its phosphotase activity. The active calcineurin dephosphorylates cytoplasmic Crz1, resulting in translocation of Crz1 to the nucleus where it binds to this part and promotes the assembly of general transcription factors at the promoter sequence.

BBa_K363003

A calcium dependent response element binding site for the Crz1 activator

This part is a binding site for the Crz1 transcriptional activator. When placed upstream of a eukaryotic promoter sequence, this sequence results in the calcium dependant transcription of downstream coding sequences given that Crz1 and the upstream signal transducing phosphotase calcineurin are constitutively expressed. The signal cascade occurs when calcineurin binds to cytosolic calcium, activating its phosphotase activity. The active calcineurin dephosphorylates cytoplasmic Crz1, resulting in translocation of Crz1 to the nucleus where it binds to this part and promotes the assembly of general transcription factors at the promoter sequence.

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New for iGEM 2010 is the groupparts tag. This tag will generate a table with all of the parts that your team adds to your team sandbox. Note that if you want to document a part you need to document it on the [http://partsregistry.org Registry], not on your team wiki.

<groupparts>iGEM010 Johns_Hopkins</groupparts>