Team:Queens-Canada/20 July 2010

Gel Electrophoresis

Thai and Yuli

Used 0.3 g agarose in 50 mL TBE. Gel run for 40 mins at 100 V.

Miniprep

Hao and Steve

pSip-1::1A3

Standard procedure followed.

PCR

Hao and Yuli

pStr-1

Digestion

Thai and Nelson

pSip-1::1A3

Cut with EcoRI and PstI.

Gel Electrophoresis

Thai and Nelson

Used 0.7 g agarose in 100 mL TBE. Gel run for 35 mins at 100 V.

PCR

Yuli and Hao

pSip-1:::eGFP, pRab-7:::eGFP, pMec-7:::eGFP, unc-54 3'UTR

Program run using HIFI with an extension time of 45 s and annealing temperature of 61 °C.

Gel Electrophoresis

Hao

pStr-1

Used 0.35 g agarose in 50 mL TBE. Gel run for 40 mins at 100 V.

PCR

Hao and Mary

pSB1A3, pSB1C3, pSB1T3

Program run using HIFI with an extension time of 40 s and annealing temperature of 65 °C.

Gel Electrophoresis

Hao and Mary

pSip-1, pRab-7, pMec-7, unc-54 3'UTR, eYFP

Used 0.35 g agarose in 50 mL TBE. Gel run for 50 mins at 100 V.