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Team:Queens-Canada/13 July 2010
From 2010.igem.org
July 13, 2010
PCR
Hao
pSra-10, pCeh-12
Program run using HIFI with an extension time of 140 s and annealing temperature of 58 °C.
Digestion-Ligation
Yuli and Thai
pSB1A3, pSip-1
Cut backbone and insert with XbaI and PstI. Ligated using T4 Ligase. Transformed using electroporation.
Gel Electrophoresis
Hao
pSra-10, pCeh-12
Used 0.5 g agarose in 70 mL TBE. Gel run for 40 mins at 100 V.
Miniprep
Hao and Mary
pBSII
Standard procedure followed.
