Team:Queens-Canada/13 July 2010

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July 13, 2010

PCR

Hao

pSra-10, pCeh-12

Program run using HIFI with an extension time of 140 s and annealing temperature of 58 °C.

Digestion-Ligation

Yuli and Thai

pSB1A3, pSip-1

Cut backbone and insert with XbaI and PstI. Ligated using T4 Ligase. Transformed using electroporation.

Gel Electrophoresis

Hao

pSra-10, pCeh-12

Used 0.5 g agarose in 70 mL TBE. Gel run for 40 mins at 100 V.

Miniprep

Hao and Mary

pBSII

Standard procedure followed.