Team:Queens-Canada/12 July 2010
From 2010.igem.org
July 12, 2010
Liquid Culture
Thai
pBSII
PCR
Hao and Basia
pOdr-10, pOdr-1, pSrb-6, pSra-10, pOsm-10, pCeh-12, pFlp-1, pStr-1
Program run using HIFI with an extension time of 140 s and annealing temperature of 55 °C.
Gel Electrophoresis
Hao
pOdr-10, pOdr-1, pSrb-6, pSra-10, pOsm-10, pCeh-12, pFlp-1, pStr-1
Used 0.7 g agarose in 100 mL TBE. Gel run for 55 mins at 100 V.