Team:Queens-Canada/9 July 2010
From 2010.igem.org
July 9, 2010
PCR
Hao
pOdr-1, pOdr-10, pSrb-6, pOsm-10, pSra-10, pCeh-12
Program run using HIFI with an extension time of 140 s and annealing temperature of 57 °C.
Gel Electrophoresis
Hao and Thai and Yuli
pOdr-1, pOdr-10, pSrb-6, pSra-6, pOsm-10, pCeh-12, unc-54 3'UTR, eYFP
Used 0.4 g agarose in 60 mL TBE. Gel run for 40 mins at 100 V.