Team:Queens-Canada/9 July 2010

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July 9, 2010

PCR

Hao

pOdr-1, pOdr-10, pSrb-6, pOsm-10, pSra-10, pCeh-12

Program run using HIFI with an extension time of 140 s and annealing temperature of 57 °C.

Gel Electrophoresis

Hao and Thai and Yuli

pOdr-1, pOdr-10, pSrb-6, pSra-6, pOsm-10, pCeh-12, unc-54 3'UTR, eYFP

Used 0.4 g agarose in 60 mL TBE. Gel run for 40 mins at 100 V.