Team:Queens-Canada/30 June 2010

PCR Stitch

Hao and Steve

pIC576, pIC518

Glycerol Stock

Thai

BBa_I13522

Socked liquid culture from 29/06/10 into -80 °C freezer.

Miniprep

Thai and Hao

BBa_I13522

Standard procedure followed.

Gel Electrophoresis

Hao and Steve

pIC576, pIC518

Used 0.35 g agarose in 50 mL TBE. Gel run for 40 mins at 100 V.

PCR

Thai

eCFP (pIC517)

Program run using TAQ with an extension time of 45 s and annealing temperature of 56 °C.

Gel Extraction

Yuli and Steve

mCherry

Extracted lane 4 from Gel on 30/06/10.

Gel Electrophoresis

Yuli

eCFP/pIC517, pIC518-1a

Used 0.2 g agarose in 40 mL TBE. Gel run for 35 mins at 100 V.

Gel Extraction

Louis and Yuli

eYFP-1a

PCR

Louis and Yuli

eYFP-1a and eYFP-2a

Program run using HIFI with an extension time of 60 s and annealing temperature of 61 °C.