Team:Queens-Canada/25 June 2010

PCR

Hao and Thai

pOdr-1, pOdr-10, pSrb-6, pFlp-1

Program run using TAQ with an extension time of 35 s and annealing temperature of 57 °C.

PCR

Hao and Thai

pSra-10, pCeh-12, pHsp-3

Program run using TAQ with an extension time of 55 s and annealing temperature of 58 °C.

Glycerol Stock

Thai

pIC576, pIC517, pIC518

Used 250µL of 80% glycerol with 750 µL of culture.

Gel Electrophoresis

Thai

pStr-220, pStr-1, pOsm-10, pOdr-1, pOdr-10, pSrb-6, pFlp-1, pSra-10, pCeh-12, pHsp-3

Used 0.4 g agarose in 60 mL TBE. Gel run for 40 mins at 100 V.

PCR

Yuli

unc-54 3'UTR

Program run using HIFI with an extension time of 30 s and annealing temperature of 63 °C.