Team:Queens-Canada/24 June 2010

PCR

Hao

pSip-1, pHsp-3, pRab-7, pMec-7, BBa_I714891

Program run using HIFI with an extension time of 30 s and annealing temperature of 57 °C.

Liquid Culture

Yuli and Thai

pIC517, pIC518, pIC576

Gel Electrophoresis

Hao, Thai and Yuli

pSip-1::eGFP, pHsp-3::eGFP, pRab-7::eGFP, pMec-7::eGFP, pHsp-3

Used 0.7 g agarose in 100 mL TBE. Gel run for 45 mins at 100 V.

PCR

Hao

pStr-220, pStr-1, pOsm-10

Program run using TAQ with an extension time of 35 s and annealing temperature of 57 °C.

Gel Extraction

Yuli and Thai

pSip-1, pRab-7, pMec-7::eGFP

Tubes stored in -20 °C fridge.

Dilution

Hao

Diluted new primers to 10 µM.