Team:Cambridge/Bioluminescence/Firefly Modelling

We modelled the Luciferin cycle as described in the [http://www.ncbi.nlm.nih.gov/pubmed/18949818 literature] We used data from [http://www.ncbi.nlm.nih.gov/pubmed/19859663 S. Inouye]'s review and the paper on [http://www.ncbi.nlm.nih.gov/pubmed/20655239 firefly luciferase inhibition] from J.M Leitão et al.

We used the [http://www.mathworks.com/products/simbiology/ Matlab Simbiology toolbox] to model the cycle. A diagram of the model we developed can be found below:

Some of the parameters could be found in the litterature, but some of the parameters (namely the diffusion of Luciferin into the cell and the regeneration rate were inferred from experimental data.)

We modelled the system both with and without the effect of the Luciferase Regenerating Enzyme (LRE.) The 4 Figures below show a comparison the model and our data for both cases.