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Team:Groningen/9 August 2010
From 2010.igem.org
Hydrophobofilm
pushing coatings into a greener future
Week 32
Cell wall isolation streptomyces- Peter
Degredation experiment-Peter
Modellers: Look up and read more articles on the ComXPA quorum sensing system. These pathways can get complicated so after reading a few articles we decided to be old fashioned and draw everything on the blackboard. Joël, Laura, Djoke
Geeske
Transformed E. coli TOP10 with:
-pSB1C3_J04450 (RFP) (plate 1 well 3A 2010 distribution)
-pSB1K3_J04450 (RFP) (plate 1 well 5A 2010 distribution)
These were used to produce pSB1C3_H and pSB1K3_EH via E.coli. Selfclosers would turn red and could easily be ignored, making isolating the backbone from gel unnecessary. Plasmids were checked by restriction.
