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From 2010.igem.org

Voigt Paper

• Type III secretion system (T3SS) - exports proteins, translocates polypeptides through both the inner and outer membranes.
• Samonella Pathogenicity Island 1 (SP-1) - tool for export proteins of interest, can be applied to the recombinant production of spider silk proteins, which form fibrils if allowed to accumulate inside of confined volume of the cell.
• They've used the whole gene DNA synthesis to construct DNA sequences that matches exactly the wild type amino acid sequence for the known fragments of silk monomers.
• The synthetic genes were designed for 3 silk genes, from the spider named Araneus diadenatas.
  • ADF-1: expressed in the minor ampullate gland, using during construction. High tensile strength, but inelastic.
  • ADF-2: expressed in the cylindrical gland, used for egg sacks, sequence similar to human elastins.
  • ADF-3: expressed in the major ampullate gland, forms extremely tough and elastic draglines which anchors the web.
• Each of the genes is expressed and exported using the Salmonella SP-1 T3SS.