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From 2010.igem.org

Week #3

20th September -24th September 2010

Monday

Planned and prepared for tomorrow's electroporation

• Depart electroporator.
• Made up LB agar plates and cloramphenicol 35ng/ml.
• We did a plan to transform Psb1C3 from plate.

We were looking for a nanodrop to quantify DNA’s concentrations

and tried to make ligations Psb1C3 with each one of primers

(our this year's biobricks).

Tuesday

Transformed DH5α competent cells, and leave 10 plates incubating 37º overnight.

• Prepared solutions for miniprep via Alcalin lisis method.

Wednsday

Results previous day transformations just two plates were completed sucessfully.

• We leave LB liquid medium at 37º overnight 4 falcon tubes each one with 30ml inoculum

Thursday

The inoculum for miniprep we prepared 6 vector’s vials

without positive results. Maybe the solutions for alcaline

lisis are not well.

• We have discused over how to quantify our cold sistem response

we gonig to use a promoter sistem, the funcional casset using J13002 as follow

• Promoter + our functional secuence + GFP

• Promoter + our functional secuence + AFP (Anty freeze protein)

• We have had a meeting with our Genomics frend’s we going to get a

colaboration on Modeling and probably at wet lab too.