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Team:NYMU-Taipei/Experiments/dddddH2O
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- Autoclaved an empty 250ml bottle and a jar of 2ml eppendorfs.
- They were then dried in the 56C incubator then left to cool down to room temperature.
- Added dddH2O into the bottle and autoclaved again to form ddddH2O.
- The ddddH2O was then passed through another filter to form dddddH2O and aloquited into the 2ml eppendorfs.
- Stored in a clean bag at -80C.
- When needed to be used, the bag is opened only in the laminar flow.
Theoretically, it's no cleaner than dddH2O though XD.
