Homology Based Capsid Shuffling

31/08/2010

• we decided to use AAV serotypes 1,2,5,6,8 and 9 as those have been described in the literature to infect pancreas cells.
  
• cap plasmids (AAV 1, 2, 5, 6, 8, 9) have been diluted to a concentration of 200ng/ul
• PCR on cap genes was performed using M13 primers as the genes are in the pBluescript vector:
  • AAV1 A
  • AAV2 A & B
  • AAV5 A & B
  • AAV6
  • AAV8 A & B
  • AAV9 A