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From 2010.igem.org

Preparing pSB1C3

Contents 1 2010-09-04 2 2010-09-05 3 2010-09-06 4 2010-09-07

2010-09-04

• Autoclaved 500ml of LB-Agar (1.2%) and left it in the 56^oC incubator overnight

2010-09-05

• Added chloramphenicol resistance @50mg/ml to the LB-agar and made two stacks of Chr-resistance plates (1:00-1:30pm).
• Transformed [http://partsregistry.org/partsdb/get_part.cgi?part=BBa_J04450 BBa_J04450] on a pSB1C3 plasmid (2009 kit 1 well 5A) onto a Chr-resistant plate (4:15-5:00pm -- waited extra long at the ice step).
  • 15uL ddddH₂0 to get the plasmid out from the biobrick plate.
  • used 2uL of plasmid in 20uL DH5α competent cell for transformation.
  • also transformed 2uL of E0240 (on pSB1A2 (amp resistance)) on a chr-resistant plate as a negative control.

2010-09-06

• Plate to 4^oC
• 3-in-1 of the BBa_J04450 plate.

2010-09-07

• Plasmid extraction of BBa_J04450 then digested with (XP)
• Gel extraction of the pSB1C3 plasmid.