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From 2010.igem.org

Contents 1 Andreas 1.1 Colony gradient PCR verification of pEX vector 1.1.1 Procedures 1.1.2 Results 2 Hassan

Andreas

Colony gradient PCR verification of pEX vector

Gradient PCR was run to optimize the colony PCR settings for designed pEX verification primers (pEXf and pEXr). Products analyzed by agarose gel electrophoresis.

Procedures

PCR settings

• Primers:
  • pEXf forward primer (CGG CTC GTA TAA TGT GTG GAA TTG)
  • pEXr reverse primer (CGT TCA CCG ACA AAC AAC AG)
• Gradient: 50°C, 55°C, 60°C
• Elongation time: 2 min 30 s
  • Expected amplicon size: 116 bp (pEXf) + 103 bp (pEXr) + 1188 bp (insert) = 1407 bp

Agarose gel analysis

• 1% agarose gel
• 2 ul sample
• 170 V, 20 min

Results

Figure to be added later.

Hassan

May the "http://www.pathguide.org" guide us through the darkness :)