Team:TU Delft/23 June 2010 content
From 2010.igem.org
LAB WORK
Ligations were performed using the digested BioBricks obtained in the early morning. The ligation protocol was in accordance with the following:
Componet | Sample |
I10341 | 3 μL |
J611XX | 4 μL |
pSB3C5 | 1.5 μL |
Ligation Buffer | 3 μL |
T4 ligase | 1 μL |
H20 | 15 μL |
We adhered to the 3:1 insert:plasmid ratio when determining the volumes of DNA added. The negative control for the ligation consisted of J61100 + pSB3C5. The mixtures were incubated overnight at 16 degrees.