Team:UNIPV-Pavia/Calendar/July/settimana1

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JULY: WEEK 1



June, 28th

Inoculum from glycerol stock for I7-1, I7-2, I8-1, I8-2, I9-1, I9-2, I10-1 and I10-2 in 5ml LB+Amp. Cultures were gown ON at 37°C 220rpm.

Inoculum of PhaP-1 and PhaP-2 in 5ml LB+Kan and ON growth at 37°C 220rpm.

We received strains and plasmid from ???Yale University???

name description growth conditions
BT340 pCP20 plasmid LB+Amp LC 30°C,LB+Amp HC 30°C
BW23473 E. coli pir+ strainLB, 37°C
BW23474 E. coli pir116 strainLB, 37°C
BW25141 E. coli pir+ strainLB, 37°C
BW25142 E. coli pir116 strainLB, 37°C
BW5328 /pAH123 helper plasmid LB+Amp LC 30°C,LB+Amp HC 30°C
MC1061 E. coli strain for integrationLB, 37°C
MG165 E. coli wil type strainLB, 37°C

June, 29th

All cultures were grown. From each culture, 5ul were aliquoted and diluted in 500ul LB+Amp for a preliminary TECAN test.

Screening of I7, I8, I9, I10

All strains received were on blotting paper disks, that were placed on an LB agar plates (with/without antiobiotic, see growth conditions), resuspended wih 80ul LB and then streaked. After plate streaking, disks were trasnferred in falcon containing liquid LB.

June, 30th

July, 1st

July, 2nd

Screening of new colonies of I7 and I8. New screening for I9, I10

This time all the clones are OK :)

June

July

week 1

week 2

week 3

week 4