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Team:Chiba/Notebook 3
From 2010.igem.org
Lux inverter
2010-10-8
transformation BBa_R0061 BBa_E0240
2010-10-9
Mini-prep BBa_R0061 BBa_E0240 digestion -------------------- BBa_R0061 20 µL 10×Buffer 3 µL SpeI 1 µL PstI 1 µL NFW 5 µL -------------------- -------------------- BBa_E0240 20 µL 10×Buffer 3 µL XbaI 1 µL PstI 1 µL NFW 5 µL -------------------- ↓ gel extraction
2010-10-11
Ligation ----------------------- BBa_R0061 (70 ng) 1 µL BBa_E0240 (100 ng) 2 µL ATP (10 mM) 1 µL 10×Buffer 1 µL T4 DNA Ligase 1 µL NFW 4 µL ----------------------- ↓ transformation
2010-10-12
Ligation ----------------------- BBa_R0061(100 ng) 1 µL BBa_E0240 (100 ng) 3 µL ATP (10 mM) 1 µL T4 DNA Ligase 1 µL dH<sub>2</sub>O 4 µL ----------------------- ↓ transformation
2010-10-13
Double transformation Strain:XL10-Gold Plasmid:Plux inv-GFP & Ptrc-LuxR ↓ not Fuluorescence
Double transformation Strain:DH10B Plasmid:Plux inv-GFP & Plac-LuxR
