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From 2010.igem.org

Contents 1 Formal Meeting - 24th June 2010 1.1 Roll call and Apologies 1.2 Previous meeting 1.3 Logo/ T-shirts 1.4 Funding 1.5 lacI/yneA Brick 1.6 Lab Training 1.7 New Brick/Modelling 1.8 Next week 1.9 Testing on concrete 1.10 Gibson Cloning 1.11 Jamboree 1.12 Other Business 1.13 Action Points

Formal Meeting - 24th June 2010

• Start Time: 3pm

Roll call and Apologies

• Absent: Harsh, Younus, Steven

Previous meeting

• Summary of igem given to new advisors, Summary of two teams progress with Lab training and lacI/yneA Brick design.

Logo/ T-shirts

• T-shirt design looked over, T-shirt co. logo needs to be added and Welcome Trust logo needs to be bigger.

|Meeting at 9am Monday morning to try to finalise design.

Funding

• Letter to Andrew Young needs to be written asking for funds.
• Neil and Jen also need to apply for funding from Civil Engineering

lacI/yneA Brick

• yneA sequence done and sent for synthesis.
• Model needs more parameters and also inclusion of ftsZ
• Cloning strategy needs more detail

Lab Training

• Summary of Lab team's week is on the wiki

New Brick/Modelling

• Trying to create one or more BioBricks to upregulate Urease in order to produce more extracellular Calcium Carbonate
• Two Bricks:

1. Upregulate rocF
2. Downregulate ahrC by antisense mRNA

• A small problem is that 168 does not export Urease
• Examined SBML shorthand for Model, still needs more parameters
• No cloning strategy as of yet

Next week

• We our on our own in terms of orgaisation, cannot use small meeting room as base, cannot be in CBCB or lab without Neil or Wendy
• Two options for next brick :

1. Quorum sensing
2. Glue/Lysis

• Neil: Think about wow factor
• Need to design primers for lacI PCR
• Insert into pSB1AT3 in front of RFP

Testing on concrete

• Would be nice to do some mechanical modelling/testing on concrete

Gibson Cloning

• Advisors/Wendy will sort out
• Steven needs to have a look at creating software to design primers for it
• Jannetta could turn this into a web service

Jamboree

• Will go on Thursday night as better for getting acclimatised
• Fly out on 4th, return on 8th (arrival may actually be on 9th)
• Neil and Jen will book flights and accommodation

Other Business

• Can submit parts to the registry even though we do not have actual DNA
• Thursday 1st July 3pm next meeting

1. Chair:Da
2. Minutes:Alan
3. Computer

Action Points

• Jannetta: Add T-shirt co. to logo, make Welcome trust bigger
• Write letter to Andrew Young
• Neil and Jen: Extract money from civ eng
• Neil and Jen: Book Fights and Accommodation