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Team:Nevada/Agrobacterium Transformations
From 2010.igem.org
Agrobacterium Transformations as an Alternative to NT Cell Transformations
Agrobacterium tumefaciens Mediated Transformation of Plant Cells
Agrobacterium tumefaciens
- Gram (-) soil bacterium
- Causative agent in crown gall disease
- Disease is the result of interkingdom transfer of DNA (i.e. bacteria --> plant)
Molecular Progression of Agrobacterium Infection
1. chemical perception of host
2. induction of virulence genes
3. formation of infection apparatus
4. preparation of T-DNA
5. export T-DNA to plant cell
6. import T-DNA to plant nucleus
7. integration of T-DNA into host genome
8. expression of T-DNA
Agrobacterium Transformation "Up Close":
Agrobacterium Transformation Overview:
