Safety

QUOTE igem safety questions: Would any of your project ideas raise safety issues in terms of:

• researcher safety,

• public safety, or

• environmental safety?

Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes,

• did you document these issues in the Registry?

• how did you manage to handle the safety issue?

• How could other teams learn from your experience?

Is there a local biosafety group, committee, or review board at your institution?

If yes, what does your local biosafety group think about your project?

If no, which specific biosafety rules or guidelines do you have to consider in your country?

Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions?

How could parts, devices and systems be made even safer through biosafety engineering?

The EPFL team was interested in several safety aspects of this project.

Is Asaia dangerous to humans?

Asaia is not dangerous for humans. When working with Asaia in the lab, no special safety measures were taken more than for working with E. coli. Because our test on infection of flies with Asaia gave only negative results, we think that Asaia has a very high specificity for the mosquito.

Can the immunotoxin or the P-proteins be dangerous for humans or mosquitoes?

All proteins that we expressed or planned to express in E. coli and in Asaia are Biological Safety Level 1. No special safety precautions have to be taken.

The toxin part of the Immunotoxin is a synthetic peptide that has been [http://www.fasebj.org/cgi/content/abstract/2/13/2878 shown] to lyse Plasmodium falciparum , but there is no danger for humans.

In order to make the biobricks for the P25 and P28 proteins we got genomic DNA from Plasmodium falciparum . There was no danger because it was just DNA. Although those two proteins come from the pathogen P. falciparum, they are not dangerous. An expert on P. falciparum ([http://doerig-lab.epfl.ch/ Prof. Christian Doerig] from the Global Health Institute at EPFL), from whom we obtained the genomic DNA, assured us that there is absolutely no risk associated with P25 and P28.

What would be the ecological impact?

We also wondered about the ecological aspect of our project. Because Asaia is not dangerous to humans, and seems to be very specific for the mosquito, introducing our modified organism in mosquito's population should not be harmful. Further test would be needed to see if it is possible to make the modified Asaia more fit to the environment than the WT Asaia. We should also make sure that the expression of the immunotoxin does not weaken the mosquito, because this would prevent our bacteria population to expand efficiently. Compared to the current solution that is killing as much mosquitoes as we can, making them resistant to malaria by an alteration of their gut flora seems less radical, and less prone to dramatic ecological changes.