"

From 2010.igem.org

Home	Brainstorm	Team	Acknowledgements	Project	Side Project	Parts	Notebook	Calendar	Protocol	Safety	Links	References	Media	Contact

Contents 1 DNA 1.1 Prepping DNA from the kit plates 1.2 Quikchange (from primers to colonies!) 1.3 Kit to Stock Plasmid 2 Bacterial Work 2.1 Transformation 2.2 O/N Culture 2.3 Preparation of Competent Cells 2.4 LB Media 2.5 Preparing Plates 3 =Glycerol Stocks 4 Assembly 4.1 Restriction Enzyme Digests 4.2 3A Assembly 4.3 Ligations 4.4 Mini Prep 5 Microscopy 5.1 Confocal Microscopy 5.2 Fluorescence Microscopy 6 Reagents 6.1 Reagents 7 Cell Staining 7.1 Rhodamine-Phalloidin/Calcofluor Staining 7.2 LIVE/DEAD® BacLight - Bacterial Viability Kit

DNA

Prepping DNA from the kit plates

Quikchange (from primers to colonies!)

Kit to Stock Plasmid

Bacterial Work

Transformation

O/N Culture

Preparation of Competent Cells

LB Media

Preparing Plates

=Glycerol Stocks

Assembly

Restriction Enzyme Digests

3A Assembly

Ligations

Mini Prep

Microscopy

Confocal Microscopy

Fluorescence Microscopy

Reagents

Reagents

Cell Staining

Rhodamine-Phalloidin/Calcofluor Staining

LIVE/DEAD® BacLight - Bacterial Viability Kit