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From 2010.igem.org

Contents 1 Andreas 1.1 Preparation of LB agar plates with antibiotics 1.2 Transformations 1.2.1 Top10 1.2.2 BL21(DE3) 1.3 Isolation of requested parts

Andreas

Preparation of LB agar plates with antibiotics

Results from 1/7 platings with non-transformed cells

• 50 ug/ml Km: No growth
• 50 ug/ml Amp & 25 ug/ml Cm: No growth
• 50 ug/ml Amp & 25 ug/ml Km: No growth

Transformations

Top10

Results from 1/7 transformations

• pSB1A3.BBa_J04450
• pSB1C3.BBa_J04450
• pSB1K3.BBa_J04450
• pSB1AC3.BBa_J04450
• pSB1AK3.BBa_J04450

Colonies inoculated in 3 ml LB with appropriate antibiotics and grown in 37°C, 250 rpm for 6 hours, and glycerol stocks were prepared. Plates stored in 4°C.

BL21(DE3)

Results from 1/7 transformations

• pEX.SOD
• pEX.yCCS
• pMA.BBa_K157011

Plates stored in 4°C.

Isolation of requested parts

Results from 1/7 platings

• BBa_J18930 (mCerulean; CFP)
• BBa_J18931 (mCitrine; YFP)
• BBa_J18932 (mCherry; RFP)

Single colonies inoculated in 3 ml LB with 100 ug/ml Amp and grown in 37°C, 250 rpm for 6 hours, and glycerol stocks were prepared. Plates stored in 4°C.