Team:Stockholm/2 July 2010
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Andreas
Preparation of LB agar plates with antibiotics
Results from 1/7 platings with non-transformed cells
- 50 ug/ml Km: No growth
- 50 ug/ml Amp & 25 ug/ml Cm: No growth
- 50 ug/ml Amp & 25 ug/ml Km: No growth
Transformations
Top10
Results from 1/7 transformations
- pSB1A3.BBa_J04450
- pSB1C3.BBa_J04450
- pSB1K3.BBa_J04450
- pSB1AC3.BBa_J04450
- pSB1AK3.BBa_J04450
Colonies inoculated in 3 ml LB with appropriate antibiotics and grown in 37°C, 250 rpm for 6 hours, and glycerol stocks were prepared. Plates stored in 4°C.
BL21(DE3)
Results from 1/7 transformations
- pEX.SOD
- pEX.yCCS
- pMA.BBa_K157011
Plates stored in 4°C.
Isolation of requested parts
Results from 1/7 platings
- BBa_J18930 (mCerulean; CFP)
- BBa_J18931 (mCitrine; YFP)
- BBa_J18932 (mCherry; RFP)
Single colonies inoculated in 3 ml LB with 100 ug/ml Amp and grown in 37°C, 250 rpm for 6 hours, and glycerol stocks were prepared. Plates stored in 4°C.