Team:Mexico-UNAM-CINVESTAV/Notebook/Week Five
From 2010.igem.org
Contents |
Week #5
04th October - 08 October 2010
Monday
- Let's picked up colonys our transformant cells and prepare all to do miniprep.
For this we used 30ml de LB liquid agar medium per falcon tube at 80μ/ml
Cloramphenicol concentration we left overnight culture to 37º
Tuesday
With a overnight inoculum we going to do a miniprep
The miniprep was done with a Quiagen Kit
Then we going to do a duble digest with EcoRI and PstI to check out our ligations
DNA | 5μl |
Buffer NB2 | 1μl |
EcoRI | 0.5μl |
PstI | 0.5μl |
H2O | 2.7μl |
BSA | 0.3μl |
Total | 10μl |