| Minutes for the formal Meeting: 24th June 2010
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| Start Time: 3pm
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| 1. Apologies
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| Harsh, Younus, Steven
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| 2. Previous meeting
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| Summary of igem given to new advisors, Summary of two teams progress with Lab training and lacI/yneA Brick design.
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| 3. Logo/ T-shirts
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| T-shirt design looked over, T-shirt co. logo needs to be added and Welcome Trust logo needs to be bigger.
| Meeting at 9am Monday morning to try to finalise design.
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| 4. Funding
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| Letter to Andrew Young needs to be written asking for funds.
| Needs to say Thanks for £5000 last year, very exciting project, interdisciplinary nature
| Neil and Jen also need to extract cash from Civil Engineering
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| Quote from Neil: You know it all know
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| 5. lacI/yneA Brick
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| yneA sequence done and sent for synthesis.
| Model needs more parameters and also inclusion of ftsZ
| Cloning strategy needs more detail
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| 6. Lab Training
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| Summary of Lab team's week is on the wiki
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| 7. New Brick/Modelling
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| Trying to create one or more BioBricks to upregulate Urease in order to produce more extracellular Calcium Carbonate
| Two Bricks: Upregulate rocF
| Downregulate ahrC by antisense mRNA
| A small problem is that 168 does not export Urease
| Examined SBML shorthand for Model, still needs more parameters
| No cloning strategy as of yet
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| 8. Next week
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| We our on our own in terms of orgaisation, cannot use small meeting room as base, cannot be in CBCB or lab without Neil or Wendy
| Two options for next brick : Quorum sensing
| Glue/Lysis
| Neil: Think about wow factor
| Need to design primers for lacI PCR
| Insert into pSB1AT3 in front of RFP
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| 9. Testing on concrete
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| Would be nice to do some mechanical modelling/testing on concrete
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| 10. Gibson Cloning
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| Advisors/Wendy will sort out
| Steven needs to have a look at creating software to design primers for it
| Jannetta could turn this into a web service
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| 11. Jamboree
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| Will go on Thursday night as better for getting acclimatised
| Fly out on 4th, return on 8th (arrival may actually be on 9th)
| Neil and Jen will book flights and accommodation
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| 12. Other Business
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| Can submit parts to the registry even though we do not have actual DNA
| Thursday 1st July 3pm next meeting
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| Chair:Da
| Minutes:Alan
| Computer:
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| 13. Action Points
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| Jannetta: Add T-shirt co. to logo, make Welcome trust bigger
| Write letter to Andrew Young
| Neil and Jen: Extract money from civ eng
| Neil and Jen: Book Fights and Accommodation
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"
