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From 2010.igem.org

BioBrick Toolbox

To construct the aimed fusion proteins, we first cloned our BioBricks into the storage vector pSEVA132 <partinfo>BBa_K422002</partinfo> (Victor de Lorenzo's lab, KanR, pBBR1 ori). All parts are sequence verified and compatible with BBF RFC28 (http://dspace.mit.edu/handle/1721.1/46721). A-parts N-Terminal fusion and C-parts C-terminal fusion. B-part is the linker that connects the two entities.

Chemotaxis BrixBox

Name	Insert	Orientation	Name	Insert	Orientation	Description	Favourite part
<partinfo>BBa_K422003</partinfo>	CheB	A-part	<partinfo>BBa_K422004</partinfo>	CheB	C-part	Demthylase: Demethylates methyl accepting chemotaxis proteins MCPs
<partinfo>BBa_K422005</partinfo>	CheR	A-part	<partinfo>BBa_K422006</partinfo>	CheR	C-part	Methyltransferase: Methylates methyl accepting chemotaxis proteins MCPs
<partinfo>BBa_K422007</partinfo>	CheY	A-part	<partinfo>BBa_K422008</partinfo>	CheY	C-part	Induces tumbling by interacting with the flagellar switch protein FliM

Anchor BrixBox

Ligth sensor BrixBox