Team:Heidelberg/Modeling/miBSdesigner

From 2010.igem.org

Revision as of 09:44, 25 October 2010 by AlejandroHD (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

BS designer for miRNA

Construction of microRNA binding sites
Sequence name

microRNA Sequence ( 5' -> 3')

Spacer (inert sequence)

Perfect Binding Site
Imperfect Binding Site with 4 nt bulge (9-12)
Personalized Binding Site

Seed sequence (1-8)

Customized MISMATCH position

Supplementary region

Customized MATCH positions (input numbers from 9 to 22)

Modify AU content

A in position -1

A in position 0

A in position 1

A in position 8

A in position 9

A in position 10

Sticky ends for integration in plasmid:

Customized: 5' 3'

Name of primer 1

Sequence of primer 1

Name of primer 2

Sequence of primer 2

div.reswrap2 {width: 550px; padding:25px; border-width:5px; margin:auto; border-style:solid; border-color:#11526f; text-align:left; background-color:#ffaf58; display:none; font-size:12pt; overflow:hidden;} .variaresult {font-size: 12pt; color: #11526f; font-weight: bold;}