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Reagents
From 2010.igem.org
Ampicillin - sodium salt Sigma A95180-5G $50.30
Chloramphenicol - Sigma C0378-5G $29.00
Kanamycin - Sigma K4000-5G $59.60
Tetracycline hydochloride - Sigma T3383-25G $47.50
USA Scientific
PO Box 3565
Ocala, FL 34478
ph 800-522-8477
fax 352-351-2057
FEIN V592244579 A
for tips 1 BG 1111-1006 Yellow beveled tips, bulk bag $10.80 1 BG 1111-2021 blue tips , bulk bag $12.95 1 BG 1111-3000 clear tips (1-10 ul) bulk bag
LabSource
7350 S. Madison Street
Willowbrook, IL 60527
ph 630 413 5662
fax 630 325 5662
FEIN V363631684 1
Vendor Note: toll free 800-545-8823
Microcentrifuge tubes
BX T56-950 1.5 ml boil proof microcentrifuge tubes $27.95
Agilent Technologies
P.O. Box 75265
Charlotte, NC 28275-0265
ph 800-227-9770
fax 302-633-8954
FEIN V770518772 E
for Taq polymerase
600630 Pfu Ultra Hotstart PCR Master Mix $357.00
This is for 100 rxn - smallest size maybe there is something cheaper.
The polymerase that I enjoy is called Phusion. It is a NEB polymerase, but a little pricey. If you are using small (<5kb) plasmids, I've had luck with Vent (also NEB). Peggy would be a good person to ask about this. -THUS SPAKE AVI
http://www.emdchemicals.com/life-science-research/novablue-gigasingles-competent-cells/EMD_BIO-71227/p_Z1Wb.s1OFS0AAAEjNxl9.zLX?attachments=TI
