User:HassanForoughiAsl/29 June 2010

From 2010.igem.org

Revision as of 12:53, 29 June 2010 by Hassanforoughiasl (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

"It has become dangerously acceptable to conclude that proteins interact directly provided that (i) the interaction is biologically plausible; (ii) the proteins are co-expressed; and (iii) an interaction is demonstrated by glutathione-S-transferase (GST)-pulldowns and/or co-immunoprecipitations (co-IPs)"... "The most obvious caveat with co-IP data is that a positive result does not imply a direct interaction between two proteins – binding could be mediated by other partners."...""

"Protein interactions: is seeing believing? J. P. Mackay et al. 2007"