Team:Yale/Our Project/Protocols

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iGEM Yale

protocols

Bacterial protocols

transformation

DNA protocols

miniprep
gel extraction
PCR purification
ethanol purification

Recipes

MOPS minimal media
SOC medium
LB agar
Triple Sugar Iron (TSI) agar
The composition of the plates were:

  • 500 mL water
  • 1.5 g Meat Extract
  • 1.5 g Yeast Extract
  • 7.5 g Pancreatic Digest of Casein
  • 2.5 g Protease Peptone
  • 0.5 g Dextrose
  • 0.5 g Lactose
  • 0.5 g Sucrose
  • 0.26 g Ferrous Ammonium Sulfate (hexahydrate)
  • 2.5 g NaCl
  • 0.15 g Sodium Thiosulfate
  • 6 g Agar
  • 12 mg Phenol Red
  • + Ampicillin

    • Copper absorbance protocols

    standard absorbance curve measurement
    copper disappearance as a function of bacterial growth curve

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