"
Team:Tokyo Metropolitan/Notebook/Pattern/2010/08/28
From 2010.igem.org
E.coli Pattern Formation Project Notebook
|
|
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Contents |
2010/08/28
1：PCR
＜Member＞
Mariko, Hitomi
＜Sample＞
・E-coli (having BBa_I732901 plasmid)
＜Protocol＞
See Protocol 2
・Tube(higher temperature/lower temperature in annealing)
- Rfp (67.5℃/66.0)
- Terminator (66.0/63.0)
Total 4 tubes.
2：Plasmid extraction from E-coli
＜Member＞
Hitomi
＜Sample＞
・transformed E-coli (8/26)
- BBa_I13521
- BBa_B0010
- BBa_B0030
- BBa_J23110
- BBa_E0040
＜Protocol＞
SeeProtocol 6
3：DNA concentration measurement
＜Member＞
Hitomi
＜Sample＞
・PCR products
＜Protocol＞
- Add 2µl of TE and 2µl of sample into the tube, and mix it gently.
- Fall in drops 1 on the measuring machine.
＜Result＞
| concentration | A320 | A260/A280 | A260/A230 | |
|---|---|---|---|---|
| RH | 565.0 | 20.24 | 1.723 | 1.175 |
| RL | 400.5 | 10.73 | 1.697 | 1.462 |
| TH | 356.0 | 8.47 | 1.622 | 1.201 |
| TL | 324.5 | 8.31 | 1.627 | 1.195 |
4：Electrophoresis
＜Member＞
Mariko, Hitomi
＜Sample＞
・PCR products
＜Protocol＞
SeeProtocol 8
＜Result＞
.JPG)
