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Team:Stockholm/19 October 2010
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Andreas
Colony PCRs
- pEX.ProtA⋅His: PA 1 & 2
- pEX.IgGp: Ig 1 & 2
Standard colony PCR settings
- Elongation time: 1:20
Gel verification
3 μl λ;5 μl sample.
λ = O'GeneRuler 1 kb DNA ladder.
Expected bands
- PA: 417 bp
- Ig: 1149 bp
Results
All four constructs confirmed.
PCR for Uppsala team
Gel verification
3 μl λ; 5 μl sample
λ = O'GeneRuler 1 kb DNA ladder.
Expected bands
- All constructs: ≈ 6570 bp
Results
Incorrect size. Send to the Uppsala team for analysis.
Nina
Agarose gel on Colony PCR
I ran agarose gels on all the colony PCR screens from yesterday to check if I had inserts.
