Here's a list of papers from which we got some ideas / inspiration and useful information

Asaia related

1. [http://www.springerlink.com/content/p21765x15336k13x/ Bacteria of the Genus Asaia: A Potential Paratransgenic Weapon Against Malaria] I (Henrike) did not manage to get this paper... could anybody send it to me please?
2. [http://www.pnas.org/content/104/21/9047.long Bacteria of the genus Asaia stably associate with Anopheles stephensi, an Asian malarial mosquito vector] isolation of Asaia from mosquitoes and expression of GFP
3. [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6VRT-4V3K67R-B&_user=164550&_coverDate=12%2F09%2F2008&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_searchStrId=1377846611&_rerunOrigin=scholar.google&_acct=C000013218&_version=1&_urlVersion=0&_userid=164550&md5=cdaba6cadd4162264dbdb63505e46eaa/ Paternal transmission of symbiotic bacteria in malaria vectors] Asaia transmission and stable Asaia mCherry strain
4. [http://aem.asm.org/cgi/content/abstract/68/5/2619 Cloning of Escherichia coli lacZ and lacY Genes and Their Expression in Gluconobacter oxydans and Acetobacter liquefaciens] Asaia plasmid

Blocking Plasmodium transmission

1. [http://linkinghub.elsevier.com/retrieve/pii/S016668510000387X Bacteria expressing single-chain immunotoxin inhibit malaria parasite development in mosquitoes] Immunotoxin against malaria parasite, composed of Shiva-1 and mouse single-chain antibody fragment against Pbs21. Expressed in E. coli, but in inclusion bodies (might be a problem for us!!!). Inclusion bodies were solubilized, refolded and purified and then shown to inhibit ookinete development.
2. [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T29-3XWJR7T-5&_user=164550&_coverDate=11%2F30%2F1999&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_searchStrId=1377833690&_rerunOrigin=scholar.google&_acct=C000013218&_version=1&_urlVersion=0&_userid=164550&md5=f323c6f84d5aa5152e389c69d5b90498/ A single-chain antibody fragment specific for the Plasmodium berghei ookinete protein Pbs21 confers transmission blockade in the mosquito midgut] 13.1 scFv single-chain antibody fragment against ookinete protein Pbs21
3. [http://www.fasebj.org/cgi/content/abstract/2/13/2878/ In vitro cytocidal effect of novel lytic peptides on Plasmodium falciparum and Trypanosoma cruzi] Shiva-1
4. Ajay K. Saxena, Yimin Wu, and David N. Garboczi, Plasmodium P25 and P28 Surface Proteins: Potential Transmission-Blocking Vaccines, Eukaryot. Cell, Aug 2007; 6: 1260 - 1265 [http://ec.asm.org/cgi/content/full/6/8/1260 Link]. Using antibodies to bind both ookinete surface proteins P25 and P28 could prevent the epithelial invasion.

Ideas

1. [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2373286/ Engineering BioBrick vectors from BioBrick parts] This might help us to make an Asaia plasmid?