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Team:TU Delft/Project/rbs-characterization/results
From 2010.igem.org
The Results
In the boxplot below the RBS strengths for the five tested members of the Anderson family are given along with the deviations from the mean. The strengths are given relative to the community RBS BBa_B0034, which has a strength of unity (1). This relationship could be built by our characterizing the community RBS [http://partsregistry.org/Part:BBa_B0032 BBa_B0032] simultaneously, which is known to have a strength of 0.3 relative to [http://partsregistry.org/Part:BBa_B0032 BBa_B0034]. Therefore in the plot below, the mean value of BBa_B0032 is fixed on 0.3.
Compared to the B0032, the tested Anderson RBS'es turned out to be surprisingly weak. All 5 of them resulted in less expression than the reference expression.
| RBS | Mean strength | Standard deviation |
| J61100 | 0.026560 | 0.005126 |
| J61117 | 0.123819 | 0.024725 |
| J61101 | 0.081210 | 0.013402 |
| J61107 | 0.019763 | 0.003963 |
| J61127 | 0.069657 | 0.007422 |
| B0032 | 0.300000 | 0.028316 |
