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From 2010.igem.org

Contents 1 Week 2 13th September - 17th 2010 Holidays in México 2 Week 3 18th September -22th 2010 2.1 Monday 2.1.1 Planned and prepared for tomorrow's electroporation 2.1.2 We were looking for a nanodrop to quantify DNA’s concentrations 2.1.3 and tried to make ligations Psb1C3 with each one of primers 2.1.4 (our this year's bricks). 2.2 Tuesday 2.2.1 Transformed Top 10 competent cells, and did 10 plates incubated overnight 2.3 Wednsday 2.3.1 Results previous day transformations only two plates were completed sucessfully. 2.3.2 We pick up colonys and left overnight to do mini prep.

Week 2 13th September - 17th 2010 Holidays in México

Week 3 18th September -22th 2010

Monday

Planned and prepared for tomorrow's electroporation

• Depart electroporator.
• Made up LB agar plates and cloramphenicol 100μl.
• Plan to transform Psb1C3 froma plate.

We were looking for a nanodrop to quantify DNA’s concentrations

and tried to make ligations Psb1C3 with each one of primers

(our this year's bricks).

Tuesday

Transformed Top 10 competent cells, and did 10 plates incubated overnight

Wednsday

Results previous day transformations only two plates were completed sucessfully.

We pick up colonys and left overnight to do mini prep.

• Prepared solutions for miniprep via Alcalin lisis method