Team:Brown/Notebook/September20

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Monday, September 20, 2010

Results of transformations

  • AraC-J13002 ligation --> 4 to 5 colonies
  • LacI-J13002 ligation --> 0 transformants

Will start overnight culture later today for AraC-J13002 ligation transformants.

J13002-LacI Ligation: remainder of DNA added for second attempt transformation of XL1B.

Overnight Culture

Started overnight culture for Arac-J13002 ligation. (3 μl Amp in 3 mL LB) picked 3 colonies.

11:05 PM - placed in 37°C.

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