Team:Northwestern/Protocol

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Tech Institute

Home	Brainstorm	Team	Acknowledgements	Project	Human Practices	Parts	Notebook	Calendar	Protocol	Safety	Links	References	Media	Contact

DNA

Prepping DNA from the kit plates

Quikchange: Primers to Colonies

Kit to Stock Plasmid

Ethanol Precipitation

New Part Design

Bacterial Work

Overnight Cultures

Preparation of Competent Cells

Preparing Plates

Glycerol Stocks

Assembly

Restriction Enzyme Digests

Plasmid Construction

Qiagen Mini Prep

Microscopy

Confocal Microscopy

Fluorescence Microscopy

Reagents

Cell Staining

Rhodamine-Conjugated Chitin Probe

Methanol Fixation

Live/Dead Baclight

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