Team:Groningen/5 July 2010

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iGEM Groningen 2010

Hydrophobofilm
pushing coatings into a greener future

Week 27

The pMASK-EH construct is cut with EcoRI and SpeI to get the fragments containing the ChpE and ChpH genes and ligate them in to the expression plasmid. A mix of both genes will be used for ligation and the clones will be checked for which gene they contain.


Mix of fragments containing ChpE and ChpH genes
alt text



Tried to cut out the insertion from pNZ8901 and replace it with a part from a Plasmid with an identical backbone, all attempts failed however.




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