Team:Harvard/vectors/vectors
From 2010.igem.org
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meet the vectors
Our vectors are a modification of the pORE agrobacterium vector system developed by Coutu et al at Agriculture and Agrofood Canada, which we obtained from The Arabidopsis Information Resource. This vector system is designed for agrobacterium mediated plant transformation, and is a binary system with the vector containing the site for foreign constructs and the appropriate flanking regions to be recognized for introduction to the plant. The virulence genes are present on a separate vector contained in the agrobacteria, separate from the TDNA.
To modify the pORE series binary vectors to fit the BioBrick standard, we used a combination of PCR mutagenesis and digestion strategies to replace the vectors' existing multiple cloning site with the BioBrick multiple cloning site. We worked with two of each of the open, reporter, and expression series vectors, creating a total of six BioBrick vectors suitable for agrobacterium mediated plant transformation.
open series click to enlarge ![]() |
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reporter series click to enlarge ![]() |
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expression series click to enlarge ![]() |
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