protocols
Standard Transformation Protocol
# Preheat SOC broth at 37 °C
# Thaw competent cells on ice
# Aliquot 50-100 μL of cells into two pre-chilled 15 mL culture tubes (one for the control, one for the actual transformation)
# Add 1-2 μL of plasmid and swirl gently
# Incubate tubes on ice for 30 minutes
# Heat pulse tubes in 37°C degree water fro 30-40 seconds
# Incubate tubes on ice for 2 minutes
# Add 500 μL of preheated SOC broth to each tube and incubate for an hour at 37°C with shaking
# Spread 100 μL of each culture on a LB agar plate containing the appropriate antibiotic(s) and incubate overnight at 37°C
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