Team:Northwestern/Protocol/Reagents

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Ampicillin - sodium salt Sigma A95180-5G $50.30

Chloramphenicol - Sigma C0378-5G $29.00

Kanamycin - Sigma K4000-5G $59.60

Tetracycline hydochloride - Sigma T3383-25G $47.50

USA Scientific PO Box 3565 Ocala, FL 34478 ph 800-522-8477 fax 352-351-2057 FEIN V592244579 A

for tips 1 BG 1111-1006 Yellow beveled tips, bulk bag $10.80 1 BG 1111-2021 blue tips , bulk bag $12.95 1 BG 1111-3000 clear tips (1-10 ul) bulk bag

LabSource 7350 S. Madison Street Willowbrook, IL 60527 ph 630 413 5662 fax 630 325 5662 FEIN V363631684 1 Vendor Note: toll free 800-545-8823 Microcentrifuge tubes BX T56-950 1.5 ml boil proof microcentrifuge tubes $27.95

Agilent Technologies P.O. Box 75265 Charlotte, NC 28275-0265 ph 800-227-9770 fax 302-633-8954 FEIN V770518772 E for Taq polymerase 600630 Pfu Ultra Hotstart PCR Master Mix $357.00 This is for 100 rxn - smallest size maybe there is something cheaper.

The polymerase that I enjoy is called Phusion. It is a NEB polymerase, but a little pricey. If you are using small (<5kb) plasmids, I've had luck with Vent (also NEB). Peggy would be a good person to ask about this. -THUS SPAKE AVI umm, did Avi say that he enjoyed a polymerase? hahaha

http://www.emdchemicals.com/life-science-research/novablue-gigasingles-competent-cells/EMD_BIO-71227/p_Z1Wb.s1OFS0AAAEjNxl9.zLX?attachments=TI

NEB 1kb DNA Ladder

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