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From 2010.igem.org

We tried to compensate for yesterdays delays. We transformed 15 Biobricks from the distribution plates!

Some PCR reactions were performed on short BioBrick inserts which would eventually save time in the long-run (compared to transforming and plasmid isolation). The BioBricks involved were J61100, J61101, J61107, J61117, J61127 and B0034.

The protocol was as follows:

-	uL
Master Mix	25
BB template (10x diluted)	1
Primers G00100 and G00101	3
H20	18}