Team:SJTU-BioX-Shanghai/safety

Safety Q&A

For iGEM 2010 we are asked to detail how we approached any issues of biological safety associated with their projects (see [1]). Here are the questions and our answers:

• Q1. Would any of your project ideas raise safety issues in terms of:
  • researcher safety,
  • public safety, or
  • environmental safety?
• Answer:
  • There is no evidence that our project causes, or will cause safety issues listed above.
    • For the most intensely focused researcher saftety, we use retroviruses which only transfects mouse cells and are unable to transfect human cells during our cell experiments. Moreover, all of our team members, no matter they are doing experiments with viral vectors or non-hazardous E.coli (DH5a or TOP10), always wear protective gears such as glasses, masks, lab coats and double-layered gloves.
    • For public or environmental safety, we have also reduced risks to minimum. All waste including air and waste water is collected and sanitized together by the laboratory's disposing system before it enters further processing factories. Strict regulations of our laboratory prevents risks of experiment matierials releasing into the environment; we have engineered multiple guarantee mechanisms in our project such as tissue-specific promoters, light-controlled switch and suicide module for E. coli. Besides, we have not yet advance our project to even animal model phase, not to mention clinical phase.