Korea U Seoul/27 September 2010

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Ligation


  • Ligation of each parts : PyodA-mAAA, PzntA-RFP and pSB1A2


Reagent Volume (μL)
10x T4 DNA ligase reaction buffer 2
T4 DNA ligase 2
Each of the digests 2 + 2 + 2 = 8
D.W. 8 / total = 20
Incubation at room temperature for 30min

and stop at 80˚C for 20min


  • Transformation to E. coli DH5α
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