Korea U Seoul/27 September 2010

From 2010.igem.org

Revision as of 13:34, 20 October 2010 by Needks (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Ligation


  1. Ligation of each parts : PyodA-mAAA, PzntA-RFP and pSB1A2


Reagent Volume (μL)
10x T4 DNA ligase reaction buffer 2
T4 DNA ligase 2
Each of the digests 2 + 2 + 2 = 8
D.W. 8 / total = 20
Incubation at room temperature for 30min

and stop at 80˚C for 20min


  1. Transformation to E. coli DH5α
Retrieved from "http://2010.igem.org/Korea_U_Seoul/27_September_2010"