Team:UNIPV-Pavia/Material Methods/Instruments

This is a oven in which plates are kept at 37 C to grow bacteria.

This is a oven where bacteria are kept to grow. In this case you can define temperature and rpm.

It is a laminar flow hood for handling highly toxic products.

It is a bath in which water is heated to high temperatures for gel extraction and heath shock during bacterial transformation.

Gel electrophoresis apparatus - Agarose gel is placed in this TBE buffer filled box and electrical field is applied via the power supply to the rear. The negative terminal is the black one while the positive is the red one. In this way DNA, that is naturally negatively charged, migrates to the positive pole.

It is a UV system with camera used to acquire gel run snapshots.

We have freezers where Dna and bacterial cultures are stocked. In particular we have two -20 C freezers to stock DNA and one -80 C freezer to stock bacteria.
We have also a fridge to stock plates and LB medium

Nanodrop is a really helpfull instrument used to measure concentration of DNA in a micro-volume of sample.

Tecan Infinite F200 is a multifunctional microplate filter-based reader with injector option, that provides high performance for the vast majority of microplate applications and research. It has been designed as a general purpose laboratory instrument for professional use, supporting common 6 to 384-well microplates. This instrument allows long incubation of the microplate, the chance to set the shaking movements, the duration of all operations, including cycles, and the dispensation of liquid material in the wells. These actions are managed by a user interface operated by i-Control software, ranging from scheduling experiments to the cleaning of injectors. We used this instrument for the following measurement techniques:

• fluorescence intensity;
• absorbance.

The Molecular Biology room is the place in which we treat DNA.
The Bacteria room is the place in which we work with bacteria. In this room the temperature is 37°C for growth of bacterial culture.

Bacteria room