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Contents 1 2010/10/ 1.1 1:PCR① 1.2 2:Electrophoresis 1.3 3：DNA Ligation 1.4 4:PCR② 1.5 5：DNA extraction from gels

2010/10/

1:PCR①

<Member>
Hitomi

<Sample>(temperature in annealing:℃)

• 9
• 1-2
• 2-10

<Protocol>
See Protocol 1

2:Electrophoresis

<Member>
nito

<Sample>
PCR products

• 9
• 12
• 210

<Protocol>
See Protocol 8

<Result>

3：DNA Ligation

＜Member＞
Hitomi

＜Sample＞

• 12
• 45
• 210

(after digestion)

＜Protocol＞
See Protocol 10

4:PCR②

<Member>
nito

<Sample>(temperature in annealing:℃)

• 7
• 9

<Protocol>
See Protocol 1

5：DNA extraction from gels

＜Member＞
nito

＜Sample＞
Digestion products

• 12
• 210

(After electrophoresis)

＜Protocol＞
See Protocol 4