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Contents 1 2010/10/04 1.1 1：DNA extraction 1.2 2：DNA Ligation 1.3 3:PCR 1.4 4:PCR

2010/10/04

1：DNA extraction

＜Member＞
nito

＜Sample＞
Digestion products (10/3)

＜Protocol＞
See Protocol 4

2：DNA Ligation

＜Member＞
nito,Hitomi

＜Sample＞

• 6-3
• 6-7
• 10-2
• 2-7
• 2-3
• 1-2
• 4-5

(after digestion)

＜Protocol＞
See Protocol 10

3:PCR

<Member>
nito,Takahiro

<Sample>(temperature in annealing:℃)
Ligated 10/3,10/4

<Protocol>
See Protocol 1

4:PCR

<Member>
Mariko,Takahiro,nito

<Sample>(temperature in annealing:℃)

• 7-8-10

<Protocol>
See Protocol 1